Flash the bin to your Pokitto, then make sure USB cable is connected and start the program. You should hear Pokitto play bongos and tambourine in 44 kHz 16 bit stereo. If not, you might need to tweak some settings on your operating system to actually hear the sound from USB input.
You can play along by tapping A, B and C buttons. Use d-pad to mute right or left channel.